FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6514467
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAG78900.021.5111623
GCCCATA79350.021.3945222
CCTTACG116550.021.3670961
CTGGAAA88300.021.3455922
ATTACGA142800.021.2660319
ATCCGAA109650.021.2658371
TCTGGAA93250.021.2632681
TCCTATA128250.021.20017210
CCATTAC142800.021.196717
GAGAAGT75600.021.18882
CGAGAAG78650.021.1789951
TGCCCAT83150.021.1714881
CGGAGAT86500.021.166122
GGACGCA104850.021.1578622
CCGAACA87050.021.139863
CCTTCTG108750.021.1090225
ACACCCA89150.021.0847767
ACGGAGA89350.021.0701051
TTACGAC144050.021.05858810
CACAGGC144850.021.0536041
Produced by FastQC (version 0.11.5)