FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6514467
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT81260612.473867777670836No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT994761.5270013648085101No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT574970.8826048240017181No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA440620.676371528169534No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA318840.4894337479950393No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT309740.47546483848947274No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC256990.3944912147072047No Hit
GTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCAGC252950.38828963290473345No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG249940.38366914745289216No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC198080.3040617137211686No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC189900.29150504561616475No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT179980.27627739921009653No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT151810.2330351815428645No Hit
GTGTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCA121010.1857557955240237No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG119820.18392909197329574No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG117220.17993797497170527No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT94580.14518455615785603No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC91900.14107063555621666No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG85150.13070908180208757No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG75700.11620290654630686No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA70390.10805181759305864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT895150.084.8792341
CGTTATC906950.083.748382
TTATCCA945350.080.517364
GTTATCC964600.078.89733
ATCCACC977950.078.628546
TATCCAC976150.078.568925
CACGCTG55800.075.07881
CACCTTC1040950.073.653449
CCACCTT1083800.070.698298
TCCACCT1087400.070.487687
CGGTATC6300.068.8053052
GCGGTAT6550.068.250271
GACGTTT4450.067.804833
GCGTTAA3100.067.2962341
GGAAGTT190300.064.49422
AAGTTTC196900.061.9007264
AGTTTCT198450.061.631395
GAAGTTT201900.060.5782553
TCTGGCG217450.059.5686659
TTCTGGC213850.059.5579578
Produced by FastQC (version 0.11.5)