FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6451335
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT78093012.104936420136298No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT939491.456272228926261No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT546470.847064987324329No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA426240.6607004596723004No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA306680.47537447675558625No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT298290.46236941656261843No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC246730.3824479739464778No Hit
GTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCAGC242640.3761082008607521No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG237840.3686678803689469No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC185230.2871188676452238No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC182710.2832126993870261No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT171660.2660844615881829No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT147150.2280923250769027No Hit
GTGTCCCAGGTCACCATCACCGGCTCCGGGAAGTAGCCCGTGGCCAGGCA116480.1805517772678058No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG114210.17703312570188962No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG112840.1749095342281869No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT90870.1408545673104869No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC88800.1376459290983959No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG80470.12473387291157567No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG72000.11160480737707776No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA66030.10235090876539507No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT865100.084.882381
CGTTATC876050.083.818892
TTATCCA911100.080.6037144
GTTATCC930750.078.9294053
ATCCACC943750.078.760816
TATCCAC943200.078.509365
CACGCTG57350.075.776881
GCGTTAA3050.073.9640051
CGGTATC6100.073.8504942
CACCTTC1007800.073.5946359
GCGGTAT6250.073.550921
CCACCTT1044850.070.9361958
TCCACCT1046850.070.853447
GACGTTT4200.068.807693
GGAAGTT186100.064.353472
GCGTGAT2550.063.4289931
AAGTTTC191200.062.2368354
AGTTTCT193500.061.8484885
GAAGTTT196650.060.7064933
CGTGATC2600.060.4791562
Produced by FastQC (version 0.11.5)