FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59640855
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGA861450.021.1941282
TGGCTGG945000.021.094541
TGGAGAG845950.020.9092395
GAGGCCG476200.020.8134827
GCAGCTA832750.020.7267866
ATTGCAG871400.020.7178363
AGGCCGA504150.020.7023378
AGTGACC871500.020.69488110
GATTGCA868550.020.6719882
CTAGCCC851400.020.67191510
AGATTGC955600.020.667961
GGCCGAA504200.020.6675649
GTAAGCC620350.020.6416285
TTGCAGC830150.020.5851844
CTGGAGA894100.020.5311134
AGCTAGC859800.020.5006548
AGAGTGA908900.020.3745178
GTGTGCG906350.020.366021
GCCGAAT511200.020.36089310
GCTGGAG927700.020.2951853
Produced by FastQC (version 0.11.5)