FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11707088
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAG713500.021.6161
ACACCCT374550.021.5815931
TGAGCCG275550.021.5414181
TCCAGGT711300.021.531683
CACCCTT358050.021.5037162
TGGGCGT357800.021.4941391
ATCCAGG714700.021.4565532
CCGTGAC713850.021.45584910
CAGGTCC712650.021.4306955
GCGCCAA216700.021.4156321
TTTACTG280000.021.3955331
AGCGCTC333050.021.37226910
CCCTTCT355400.021.3502864
TCCGTGA717950.021.348559
CCTTCTT361700.021.3372785
ACCCTTC361050.021.3330363
GTCCGTG719600.021.31038
AGGTCCG716900.021.3100456
GATTGAT174900.021.306332
GATGAAG151000.021.3009193
Produced by FastQC (version 0.11.5)