FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47246686
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGA666700.021.196112
TGGCTGG729050.021.154931
GAGGCCG379550.020.8687827
TGGAGAG656750.020.8679185
ATTGCAG675000.020.7209853
CTAGCCC655650.020.71441510
AGTGACC675000.020.67131610
GCAGCTA644400.020.6669226
GATTGCA676000.020.642062
GGCCGAA403850.020.601649
AGATTGC747000.020.5861051
GTAAGCC489350.020.57175
TTGCAGC642300.020.5325934
AGGCCGA406350.020.491138
AGCTAGC665750.020.4100728
CTGGAGA695850.020.395534
GTGTGCG708450.020.3639371
AGAGTGA710700.020.2132598
GCTGGAG720100.020.2067553
GCCGAAT411800.020.19323310
Produced by FastQC (version 0.11.5)