FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9540370
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCG234250.021.6009031
AATCCAG584200.021.5923841
TCCAGGT582400.021.5468063
ACACCCT316700.021.5393071
CACCCTT300800.021.4890962
TGGGCGT290600.021.4861351
ATCCAGG584600.021.4853922
CCGTGAC584900.021.48258210
TTTACTG222900.021.3977031
CAGGTCC586900.021.3703525
TCCGTGA588550.021.3436349
GAGCCGA180300.021.3396842
AGGTCCG588100.021.320136
GCGCCAA174750.021.318561
CCCTTCT299300.021.3165054
TGTAGCG221200.021.2935077
TAGCGCT272650.021.2895879
GTCCGTG590400.021.2804818
CGATTGA153700.021.274681
GGTCCGT588950.021.2693717