FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35243474
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTGG539100.021.1927851
GGCTGGA493650.021.1660962
GAGGCCG294500.020.9429137
TGGAGAG487000.020.8355835
ATTGCAG509900.020.769293
GTAAGCC361300.020.7270055
GGCCGAA310050.020.72269
GATTGCA509550.020.7197252
AGGCCGA310600.020.7177728
CTAGCCC497450.020.70930310
GCAGCTA486400.020.6645076
AGATTGC561150.020.6604841
AGTGACC501300.020.62484710
TTGCAGC487300.020.5248374
AGCTAGC504500.020.4569668
GTGTGCG533350.020.3696211
GCCGAAT315600.020.36166610
CTGGAGA516800.020.3470754
CGGCGCA256050.020.2220929
AGAGTGA526700.020.1731248
Produced by FastQC (version 0.11.5)