FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005394996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005394996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9791304
Sequences flagged as poor quality0
Sequence length28
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAG601350.021.5875631
TCCAGGT598500.021.5661223
ACACCCT326300.021.5344811
TGGGCGT294050.021.5126381
ATCCAGG601700.021.4725972
CCGTGAC601550.021.47243310
TGAGCCG237500.021.4367241
TTTACTG232750.021.434551
AGTGTAG220000.021.4267025
CACCCTT312050.021.4247672
CAGGTCC602850.021.388615
AGCGCTC277800.021.38136510
GATGAAG131300.021.3733633
TCCGTGA603950.021.3725369
CCCTTCT308500.021.342054
GTCCGTG605600.021.3179388
TAGCGCT276350.021.3144429
AGGTCCG605100.021.288076
GAGCCGA182950.021.2848572
ACCCTTC314550.021.263813
Produced by FastQC (version 0.11.5)