FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56452126
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTGG3212850.021.60299
TGCTGGC3211800.021.58119410
GCCATGC3189200.021.5673246
GGCCATG3189250.021.5571945
CATGCTG3224600.021.5555678
CGGACGT2496250.021.5161131
AGGCGAA1286350.021.4438810
CGTGTCG2447450.021.4331975
GGACGTG2460450.021.3803062
ACGTGTC2451800.021.371394
ACAGGCC3241700.021.3556752
GTCGCGA2469150.021.3501828
TCGCGAA2472950.021.3209329
CAGGCCA3241700.021.3119013
CGCGAAA2490700.021.28911410
TCACTCG1202500.021.2847887
AGGCCAT3236350.021.2781314
GTGTCGC2459350.021.273716
ACTCGAC1221500.021.2675849
CACAGGC3319550.021.2528251
Produced by FastQC (version 0.11.5)