FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6972730
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAG426000.021.6580161
TCCAGGT424900.021.5720373
TGAGCCG168650.021.569581
TGGGCGT205450.021.5407661
ACACCCT232700.021.5196721
ATCCAGG427050.021.4733582
CCGTGAC427350.021.4275810
AGTGTAG160650.021.4266095
CAGGTCC426900.021.4194365
TTTACTG163650.021.415031
CACCCTT224200.021.3932362
TCCGTGA428500.021.3854759
CCTTCTT224050.021.383165
CCCTTCT221350.021.3706424
GATGAAG86850.021.3684943
AGCGCTC205150.021.3609410
GCGCCAA132300.021.357991
TGTAGCG163450.021.3327547
AGGTCCG428500.021.3314156
GTCCGTG430400.021.2885138