FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22181420
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGGC1268050.021.63791810
ATGCTGG1271100.021.6084969
GCCATGC1260800.021.5800066
CATGCTG1274800.021.5751178
GGCCATG1260900.021.561625
CGGACGT991750.021.5124071
AGGCGAA508100.021.44976610
GGACGTG975400.021.4343032
CGTGTCG973250.021.4319235
ACAGGCC1278050.021.3927842
CAGGCCA1278800.021.344973
ACGTGTC975900.021.341044
GTCGCGA982350.021.3322818
TCGCGAA982750.021.3280749
AGGCCAT1278550.021.3087084
TCACTCG464000.021.2984647
CACAGGC1307000.021.2724251
CGCGAAA992250.021.26355610
GTGTCGC978000.021.253696
ACTCGAC471550.021.229869
Produced by FastQC (version 0.11.5)