FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8538541
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCAG530400.021.6185721
ACACCCT287850.021.5630911
TCCAGGT528850.021.5567133
TGGGCGT255800.021.5290411
ATCCAGG531650.021.4559842
CCGTGAC531950.021.42406710
TTTACTG198050.021.4120371
TGAGCCG204300.021.3871361
CAGGTCC532250.021.3776725
GATTGAT135100.021.3712352
CACCCTT275250.021.3708972
TCCGTGA533900.021.3581819
AGCGCTC245800.021.3545110
GATGAAG115350.021.3533543
ACCCTTC275800.021.3357493
AGCGTAT148350.021.3167171
CCCTTCT272600.021.3117894
AGGTCCG533800.021.3065456
GCGCCAA159900.021.3046111
GTATAGA146450.021.3032764
Produced by FastQC (version 0.11.5)