FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7818144
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA198350.021.5715610
GATGCTA174800.021.5710771
TCTAGAA281200.021.5682835
AGGTGAG285250.021.563242
GGTGAGT285850.021.5487633
GGCCACC276750.021.5458832
AGGCCAC281100.021.4863721
ACACTGA165500.021.4805721
GCTACAA173650.021.4728164
ACCCACT175100.021.4713021
AGAACAA291150.021.4696548
GCAACGA291400.021.46255510
CTAGAAC282950.021.4543256
GAGGTGA291650.021.4521141
TGGCCCT199700.021.4202279
ATGCTAC174050.021.417562
CACTGAT160700.021.4171752
AACCTAG215100.021.4109062
GCCACCA279100.021.3881133
TGCTACA174300.021.3805313
Produced by FastQC (version 0.11.5)