FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48708058
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGGC2772250.021.64135410
ATGCTGG2779750.021.617399
GCCATGC2757550.021.5877196
CATGCTG2787650.021.5833748
GGCCATG2760850.021.5546555
CGGACGT2131650.021.5174661
AGGCGAA1136150.021.46037710
CGTGTCG2094550.021.4490625
GGACGTG2098600.021.4323042
ACAGGCC2799500.021.3832022
ACGTGTC2096100.021.370754
GTCGCGA2113250.021.3599538
TCGCGAA2115550.021.3481489
TCACTCG1051750.021.3036967
CAGGCCA2805550.021.3018043
CGCGAAA2136100.021.27717410
CCCAGTT1173650.021.2769131
AGGCCAT2802400.021.2762874
GTGTCGC2105150.021.2659026
ACTCGAC1069500.021.256049
Produced by FastQC (version 0.11.5)