FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7703067
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGAG284500.021.6284962
GGCCCTA194150.021.6199310
GGTGAGT284100.021.6047443
GATGCTA175700.021.5937141
GCAACGA288950.021.58458510
TGAGTGC284850.021.5129625
CACTGAT154450.021.5011352
ACCCACT179850.021.4930061
GAGGTGA291550.021.4913041
ACACTGA159950.021.4504811
TGCTACA175000.021.4402483
AACCTAG216700.021.4311492
GGCCACC269600.021.4243812
TCTAGAA262850.021.4220245
GAGTGCA286450.021.4196826
TGATCGC154200.021.4145835
TGGCCCT196100.021.4049459
AGTGGCC196100.021.3937267
GTGGCCC196450.021.3836068
TCAATCT272650.021.379341
Produced by FastQC (version 0.11.5)