FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41780096
Sequences flagged as poor quality0
Sequence length28
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGGC2369400.021.64584710
ATGCTGG2372950.021.641749
GCCATGC2356100.021.598956
CATGCTG2381200.021.5959648
GGCCATG2358100.021.5699025
CGGACGT1819750.021.5216081
CGTGTCG1789950.021.462125
AGGCGAA963700.021.45971310
GGACGTG1793750.021.4319842
ACAGGCC2390700.021.4050672
ACGTGTC1795100.021.3643914
GTCGCGA1810600.021.333258
TCGCGAA1810850.021.3289879
CAGGCCA2395550.021.327293
AGGCCAT2393500.021.2890264
TCACTCG889250.021.2840297
CGCGAAA1826650.021.28119510
CACAGGC2449400.021.2732751
CTCTGTG995050.021.2697372
ACTCGAC903650.021.2595049
Produced by FastQC (version 0.11.5)