FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28877100
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC779150.021.4134253
GGACAGA1251600.021.3252751
CACTGAT781550.021.3239382
TGATCCG759550.021.3052985
GACAGAT1236400.021.2987562
ACCCGAG335200.021.21889710
GATCCGC584400.021.2085956
CCTTCGA599200.021.2056121
TCCGTAC776100.021.1972628
ACAGATC1244750.021.1875673
CGATCCG583100.021.1694075
ACACTGA810600.021.1631581
ATCATCG1252150.021.1576947
TTCACCC336250.021.1493647
TCCGCAG579400.021.0986618
GATCCGT768200.021.0851526
TTACCGG535950.021.0812512
ATCCGTA768600.021.0764187
ATCCGCA589650.021.0732357
CGAATCC502000.021.065399
Produced by FastQC (version 0.11.5)