FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28877100
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415400.1438510099698377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA681750.078.165619
CGGGTTA465250.077.297359
GAGGTTA408850.077.087459
AGGGTTA431000.074.713319
CAGGTTA305800.074.157719
TTTTCCG308100.073.231689
TGGGTTA319000.072.8610159
ACGGTTA312450.072.02179
GCGGTTA503400.071.701119
GGTTCCG355650.071.494759
CCGGTTA370700.069.9220359
CGTTCCG230150.069.726689
CTGGTTA215100.069.705359
TATTCCG239150.068.7553259
GTGGTTA355950.068.712279
GGGTATA176000.068.553679
CATTCCG153600.067.483659
TGTTCCG307950.066.891489
TAGGTTA198600.066.872699
GATTCCG240100.066.4831169
Produced by FastQC (version 0.11.5)