FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28785476
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC782000.021.4014023
GGACAGA1259550.021.3688641
CACTGAT782950.021.3527322
TGATCCG761900.021.2957945
GACAGAT1247100.021.2818412
GATCCGC575900.021.2429966
ACAGATC1252350.021.2266483
CGATCCG574200.021.2139475
ATCATCG1259450.021.1717857
CCTTCGA590350.021.1688481
ACACTGA812700.021.1647931
TCCGTAC780850.021.1603688
ATCCGCA581050.021.1471377
ACCCGAG336000.021.12922510
TTCACCC338750.021.1236467
GATCCGT769050.021.1106746
TCCGCAG572700.021.1001768
GATCATC1259750.021.0719096
AGATCAT1261550.021.0714845
AATTCCC296100.021.06805410
Produced by FastQC (version 0.11.5)