FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6486733
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA168500.021.58072310
TGGCCCT168650.021.5615279
GGCCACC231450.021.5426062
GGTGAGT230700.021.526823
CACTGAT131950.021.4903372
TCTAGAA228400.021.483215
TTTCTAA139750.021.4636212
GATGCTA147250.021.4610041
ACCCACT146250.021.4573251
GTGGCCC169650.021.4538848
AGGTGAG231250.021.451842
TGAGTGC230500.021.4401825
AATGAAG142950.021.4294382
GAGGTGA235400.021.42411
GACACGC143100.021.414661
TGCTACA145850.021.4105913
AACCTAG178500.021.407272
GCAACGA236500.021.38458410
TCAATCT234850.021.3806021
GCCACCA233000.021.3756943
Produced by FastQC (version 0.11.5)