FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28785476
Sequences flagged as poor quality0
Sequence length91
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG403970.14033813441195137No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA685150.078.1695259
CGGGTTA464400.077.256829
GAGGTTA415000.076.212339
AGGGTTA435800.074.4969
TTTTCCG307150.073.625199
CAGGTTA305900.072.939629
TGGGTTA318750.072.839139
ACGGTTA317700.071.581619
GCGGTTA494650.071.466849
GGTTCCG362350.071.0768369
CCGGTTA379250.069.803389
GGGTATA165350.069.757259
CGTTCCG230100.069.705819
CTGGTTA220100.069.377859
GTGGTTA349700.069.345759
TATTCCG234550.068.528279
CATTCCG151600.067.730069
TGTTCCG304400.066.7230769
GATTCCG241000.066.535519
TAGGTTA195350.066.33279
Produced by FastQC (version 0.11.5)