FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71690575
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC1937450.021.4254343
GGACAGA3156750.021.3348881
GACAGAT3113250.021.308862
CACTGAT1948550.021.3007012
TGATCCG1886750.021.2961145
ACAGATC3130850.021.2279643
CGATCCG1444350.021.2084855
ATCATCG3144400.021.1990537
TCCGTAC1929950.021.1901688
ACCCGAG822850.021.18521910
CCTTCGA1484500.021.1816641
GATCCGC1452600.021.1779756
TTCACCC822700.021.1476387
GATCCGT1904700.021.128756
ACACTGA2016700.021.1282441
AATTCCC734000.021.11847510
GATCATC3144850.021.1040086
ATCCGCA1464900.021.0850837
CCGTACA1940750.021.0830149
TCCGCAG1438400.021.0721158
Produced by FastQC (version 0.11.5)