FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71745403
Sequences flagged as poor quality0
Sequence length28
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC1943300.021.4432263
GGACAGA3152800.021.3641871
CACTGAT1953500.021.321342
TGATCCG1895100.021.3171635
GACAGAT3117850.021.2732892
ACAGATC3128700.021.2431223
ACCCGAG813500.021.22178310
CGATCCG1453300.021.211045
TCCGTAC1938250.021.204968
TTCACCC815150.021.1956527
ATCATCG3148850.021.1916587
GATCCGC1460150.021.1861066
CCTTCGA1496500.021.1588651
ACACTGA2021800.021.1579421
AATTCCC728700.021.12559510
GATCCGT1915750.021.1241286
ATCCGCA1469350.021.1228317
TCCGCAG1447300.021.1079928
GATCATC3148150.021.0938936
TTAACTC1847600.021.0713671
Produced by FastQC (version 0.11.5)