FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9839026
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCAC408850.021.6263852
TGGTTCC534900.021.6165471
ACGCAGC333300.021.6090641
CCTATTA289750.021.5862981
CGCAGTT339700.021.5619052
AGTGTCA414400.021.5256521
GCAGTTC340500.021.5220413
CAGATTG403150.021.5212968
GTCACAG409700.021.5108594
ACTGATG176950.021.5095331
CGCAAAC320700.021.5089999
CGCCTGT343350.021.505899
TCGCCTG346000.021.4556068
AAAGATG303300.021.4539241
CGCAGCC269700.021.4422262
ACAGATT404800.021.4335737
GTTCGCC342350.021.428016
GCGCAGT358700.021.4183121
TCACAGA405850.021.4032735
TCGCAAA322100.021.3950258
Produced by FastQC (version 0.11.5)