FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9839026
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT171511917.431796602631195No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1716331.7444104731504928No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1187241.2066641555780013No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT818130.8315152333167937No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA777700.7904237675558536No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG593170.6028747154443945No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC485470.4934126609686772No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT454490.46192580444446435No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC439490.44668039295759565No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT392530.39895209139603854No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT338050.3435807568757314No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC275380.27988542768359387No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG271840.27628751057269285No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268450.27284204757666053No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC249660.25374462878744297No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAATTTATT233500.23732023881225642No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT226100.2297991691454012No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG224950.22863035426474126No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC222370.22600814348899984No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC218050.22161746498078166No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT217310.2208653580140961No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC199000.20225579239245833No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT190990.19411474265847048No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG177210.18010929130586706No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC176840.1797332378225243No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA171240.17404161753409333No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG147940.15036041169115724No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC143310.14565466134554375No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA139290.14156889106706294No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATATAAGTTATT131770.13392585810831276No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT130310.13244197139025754No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT115360.11724737794167837No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG112330.11416780482133089No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT106340.10807980383424132No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG104160.1058641373648164No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG101990.1036586345030494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2060350.084.79141
CGTTATC2085250.083.629462
TTATCCA2193650.079.781684
CACGCTG159150.078.695811
TATCCAC2264550.078.638875
ATCCACC2279000.078.306226
CGGTATC44050.078.150882
GTTATCC2297950.076.08843
CACCTTC2378600.074.662069
TCCACCT2445500.072.753397
CCACCTT2467350.072.021268
GCGTGAT12000.070.879671
CGTGATC12050.069.4822542
CGTTATA8700.067.4146962
GCGGTAT51300.067.397861
GCGTTAA8900.067.37551
ACGCTGC187100.067.328542
AAGCAGT43200.065.760581
GCGATAT6600.065.080421
GAGTTAT13450.064.819331
Produced by FastQC (version 0.11.5)