FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56092039
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC1521950.021.4236623
GGACAGA2458800.021.3610941
CACTGAT1527150.021.342352
GACAGAT2428600.021.3214442
TGATCCG1484300.021.2827765
ACAGATC2440700.021.2408453
CGATCCG1127500.021.2098795
ACCCGAG637250.021.20959310
TCCGTAC1514950.021.20028
ATCATCG2454500.021.1999037
ACACTGA1580750.021.1685371
CCTTCGA1156300.021.1628631
GATCCGC1136600.021.1512366
ATCCGCA1141800.021.133547
TTCACCC642250.021.1112587
GATCCGT1498400.021.1066156
AATTCCC562200.021.1045310
GATCATC2454450.021.1007676
TCCGCAG1123900.021.0953258
CGAATCC962000.021.091139
Produced by FastQC (version 0.11.5)