FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9451293
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATTA267800.021.6429041
ACGCAGC328050.021.6221521
GTGTCAC395750.021.566732
CGCAGTT320400.021.5585882
AGTGTCA400300.021.543771
TGGTTCC522950.021.5426271
GCAGTTC321200.021.5331023
GATGGTA279950.021.5049674
CGCAGCC262700.021.5031642
CAGATTG382950.021.4932258
GTCACAG391800.021.4823474
AAAGATG288600.021.4387281
CGCAAAC311450.021.4377639
ACAGATT384900.021.4357667
GTTCGCC322000.021.4356636
CGCCTGT323450.021.4244619
GCGCAGT339100.021.4213331
CTCGCAA311600.021.399327
CACAGAT385950.021.3973966
TCGCCTG325950.021.3952148