FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56278147
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC1513500.021.433883
GGACAGA2473600.021.343131
CACTGAT1520950.021.3138482
GACAGAT2441850.021.2782232
TGATCCG1479350.021.250135
ACCCGAG647700.021.23753510
ACAGATC2453150.021.2078043
TCCGTAC1510250.021.1878628
TTCACCC650600.021.1804377
CGATCCG1117600.021.1637655
ATCATCG2464700.021.1624877
GATCCGC1127900.021.1235756
ATCCGCA1129800.021.097457
CCTTCGA1153550.021.0967251
ACACTGA1582750.021.0934221
GATCCGT1493300.021.0678026
TCCGCAG1112650.021.067778
AGATCAT2469250.021.0677225
GATCATC2465900.021.0557356
TTAACTC1459550.021.0497041
Produced by FastQC (version 0.11.5)