FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7055350
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCAC302650.021.6087862
ACGCAGC248600.021.599751
TGGTTCC392300.021.5891481
CCTATTA211300.021.579651
CAGATTG297750.021.5417618
CGCCTGT248100.021.5350659
GCAGTTC247900.021.5307143
CGCAGTT247500.021.5130542
AAAGATG220200.021.5081231
TGATGCA122300.021.474973
CTGATGC122200.021.4613022
TCGCAAA234150.021.4606298
AGTGTCA307800.021.4586091
GTCACAG302850.021.4371384
ACTGATG127050.021.4314481
CGCAGCC202350.021.4285052
CTTACTC318350.021.4257552
CGCAAAC234950.021.4062799
GCGCAGT261850.021.3989181
CTCGCAA234700.021.396287
Produced by FastQC (version 0.11.5)