FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48385319
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC1315500.021.4164333
CACTGAT1318300.021.3576012
GGACAGA2098400.021.3193441
GACAGAT2067900.021.3168142
TGATCCG1279900.021.2797855
TCCGTAC1305650.021.2165378
CGATCCG980400.021.2109135
GATCCGC986550.021.1899766
ACAGATC2083150.021.1838873
CCTTCGA1009050.021.1834181
ATCATCG2091250.021.1661367
ATCCGCA990100.021.1578887
TTCACCC552700.021.1289567
ACACTGA1369250.021.1196351
ACCCGAG553850.021.11884310
TCCGCAG974450.021.1128168
CGAATCC832150.021.1123649
AATTCCC498200.021.10689710
ATCCGTA1294500.021.089177
CCGTACA1314100.021.0868029
Produced by FastQC (version 0.11.5)