FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761104
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTCC372450.021.632411
ACGCAGC231350.021.6167811
AGTGTCA288650.021.578771
GTGTCAC286200.021.5772252
CCTATTA202750.021.5732861
GCAGTTC233800.021.5506233
GTTCGCC233950.021.511716
CGCCTGT235300.021.5004659
CGCAAAC221550.021.4895739
TTCGCCT234100.021.4838317
CGCAGTT234250.021.4794642
AAAGATG209200.021.4560011
CAGATTG278300.021.4466068
TCGCCTG237350.021.4445088
GCGCAGT246600.021.432961
CTTACTC312300.021.421772
GTCACAG284200.021.3922234
CGCAGCC187200.021.3917432
TCACAGA279950.021.3909155
CTATTAG203100.021.3501992