FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761104
Sequences flagged as poor quality0
Sequence length91
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT108624916.066148368668788No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT1158251.7131078001462483No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA737011.0900734554593452No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA497040.7351462128078491No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT493480.7298808005319841No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG392490.5805117034141171No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAATTTATT348020.5147384214175673No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT286120.4231853259467684No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC269500.39860354167011774No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC266300.3938705868154076No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT250490.3704868317363555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG213590.31590994606797945No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT198830.294079191800629No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG170320.25191152214194606No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC167150.24722293873899884No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC159820.2363815140249285No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC143830.21273153023529884No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143530.21228781571766978No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG142780.21117852942359708No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC139710.20663785085985958No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC130600.19316372000785673No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATATAAGTTATT129590.19166988113183883No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT118730.17560741559366636No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC114870.1698982888001723No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA112230.16599360104503644No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG106670.1577700919849776No Hit
GCGGTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT96070.14209217902875032No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA90940.13450466077729317No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC89230.13197548802680745No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG88960.1315761449609413No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT87170.12892864833908782No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGAGAGAAGTTATT86090.12733127607562317No Hit
AGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCT76990.11387193570754126No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG73720.10903544746538435No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCGGGGATAGAAGTTATT70240.1038883590608871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1381200.084.8172151
CGTTATC1394900.083.707352
TTATCCA1471600.079.699734
CACGCTG107500.078.357261
TATCCAC1516550.078.267855
ATCCACC1522350.078.100876
CGGTATC24300.076.9539952
GTTATCC1540100.075.9782643
CACCTTC1587100.074.3976449
GCGTGAT9250.073.54981
TCCACCT1632300.072.5015267
CGTGATC9050.072.319752
CCACCTT1645050.071.849188
CGTTATA8000.071.718062
CGTTAGC4200.070.832662
GCGTTAG4300.070.209051
CGTTAAC6750.068.6289752
GAGTTAT12050.068.457011
CGTAATC6050.068.139842
GCGGTAT28950.065.947911
Produced by FastQC (version 0.11.5)