FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48558025
Sequences flagged as poor quality0
Sequence length28
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC1291550.021.4065863
CACTGAT1293200.021.3607392
GGACAGA2126300.021.3191281
GACAGAT2098800.021.2871972
TGATCCG1259150.021.2547195
ACCCGAG554900.021.21761510
ACAGATC2111500.021.2053073
CGATCCG956450.021.183035
TCCGTAC1289300.021.1796888
ATCATCG2120900.021.1770467
TTCACCC558900.021.1586027
CCTTCGA987150.021.1493021
ATCCGCA966100.021.1362557
GATCCGC963450.021.1295836
TTAACTC1248850.021.1199261
ACACTGA1344700.021.1177641
AATTCCC496850.021.1111110
GATCCGT1270300.021.1027876
TCCGCAG953200.021.0807618
GATCATC2119300.021.0786046
Produced by FastQC (version 0.11.5)