FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430507
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGC262100.021.7061311
CCTATTA217550.021.6634431
GTGTCAC315750.021.5950532
TGGTTCC407550.021.5759181
CAGATTG311000.021.55418
CGCAAAC243450.021.5308329
AGTGTCA321250.021.4928761
CGCAGCC213200.021.4749012
TCGCAAA244150.021.4736068
CTATTAG217500.021.4455072
GTTCGCC255150.021.4400986
CGCAGTT254400.021.4361932
GTCACAG317750.021.4321084
AAAGATG228000.021.4244751
CGCCTGT255850.021.4201269
TCGCCTG257500.021.4195468
TGATGCA132850.021.4170783
GCAGTTC255100.021.4016193
ACTGATG137850.021.3987731
GATGGTA223150.021.3950044
Produced by FastQC (version 0.11.5)