FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38386490
Sequences flagged as poor quality0
Sequence length28
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC6417450.021.833261
CCACACT6385950.021.8088382
ATTTCTG2024050.021.688261
TCTGAGC1977750.021.661854
TGAGCTG1968800.021.6586076
ACACTCT6435800.021.6458664
AGCTGAA1999400.021.6319228
CTGAGCT1978350.021.6185515
TTCTGAG1991650.021.600723
CTTCATG6466600.021.588339
GAGCTGA1980300.021.584497
TTTCTGA1974950.021.583422
TCTTCAT6471100.021.5615868
CTCTTCA6447050.021.5522737
ACTCTTC6450200.021.54846
CTGAAAT1990350.021.54624410
GCTGAAA1992850.021.5319089
CACTCTT6459150.021.500835
CACACTC6500500.021.4917093
GCAGAGA1082000.021.443914
Produced by FastQC (version 0.11.5)