FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38386490
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG595110.15503110599588554No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTCA1077400.080.850879
TCTCTCA536950.077.999929
GTTCTCA579400.077.639689
ATTCTCA691500.077.572439
TGTCTCA823800.077.438919
TATCTCA613300.076.812899
CTTCTCA464650.074.487479
GGTCTCA420600.073.04339
AGTCTCA368350.072.386119
GCTCTCA273900.071.451119
ACTCTCA329250.069.675249
TTTTCTC437500.069.0949258
CATCTCA219500.067.087279
CGTCTCA316700.066.9747249
TATTCTC316650.066.6631858
AATCTCA366700.066.17549
GGTTCCG270950.065.547279
TTATCTC284600.065.5244148
ATTTCTC321550.065.343348
TTTTCCG237150.064.549379
Produced by FastQC (version 0.11.5)