FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6916235
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGC247600.021.6207491
CCTATTA203500.021.6157631
GCAGTTC236600.021.5620043
GTGTCAC292150.021.5583952
CGCAAAC228100.021.532659
TGGTTCC383350.021.5174881
CGCAGTT237050.021.5112482
AGTGTCA296000.021.4925461
CAGATTG283800.021.4839868
TCGCCTG239950.021.4821768
AAAGATG212700.021.468451
GTTCGCC237150.021.4622026
TCGCAAA229000.021.4528278
GATGGTA208600.021.4423984
CGCCTGT239350.021.416569
GTAAACG212300.021.3997768
GCGCAGT249850.021.3995781
GATGCAC116550.021.382634
CTGATGC116500.021.3790592
CTATTAG203650.021.3756222
Produced by FastQC (version 0.11.5)