FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38728827
Sequences flagged as poor quality0
Sequence length28
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC6462550.021.8484211
CCACACT6432900.021.8126372
ATTTCTG2046750.021.7083911
TCTGAGC1996050.021.7040924
AGCTGAA2024000.021.6352148
TTCTGAG2013150.021.6302783
TGAGCTG1994750.021.6266986
ACACTCT6495000.021.620664
TTTCTGA1998200.021.6016162
CTGAGCT2005350.021.5941145
GAGCTGA2004350.021.5888337
CTTCATG6525050.021.5762449
CTGAAAT2009900.021.57573710
TCTTCAT6522250.021.5672938
CTCTTCA6500000.021.5488877
ACTCTTC6507550.021.5336636
GCTGAAA2015400.021.5305029
CACTCTT6517750.021.4807225
CACACTC6556800.021.4693873
GCAGAGA1071000.021.450544
Produced by FastQC (version 0.11.5)