FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6730608
Sequences flagged as poor quality0
Sequence length28
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGC234300.021.6730371
TGGTTCC370800.021.6208531
CGCAGTT226950.021.5818582
GTGTCAC287050.021.5814462
AGTGTCA290750.021.5661261
CCTATTA200900.021.5626771
GCAGTTC227600.021.553063
CGCAAAC222350.021.5013929
CAGATTG279100.021.4955718
TCGCCTG230950.021.472198
AAAGATG210250.021.4633481
GTCACAG284350.021.4390584
GTTCGCC228300.021.4372046
TCGCAAA223200.021.4342928
CGCAGCC189950.021.4233442
CTATTAG200150.021.4161422
CGCCTGT230050.021.4127719
TCACAGA281350.021.4018485
ACAGATT280750.021.3927427
GATGGTA207250.021.380454