FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34832559
Sequences flagged as poor quality0
Sequence length28
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC5791350.021.8442291
CCACACT5765750.021.8133242
TCTGAGC1801700.021.7144184
ATTTCTG1855000.021.692351
TGAGCTG1801650.021.6527126
AGCTGAA1826700.021.6427128
CTGAGCT1806900.021.6415465
TTTCTGA1804450.021.6275462
ACACTCT5820200.021.6215864
TTCTGAG1818750.021.6149443
GAGCTGA1809200.021.6082527
CTTCATG5843500.021.5711779
CTGAAAT1819050.021.55413210
TCTTCAT5843600.021.5529238
CTCTTCA5821450.021.551987
ACTCTTC5829150.021.5323126
GCTGAAA1821950.021.5319029
CACTCTT5841250.021.474155
CACACTC5874950.021.4695823
GCAGAGA965650.021.446534
Produced by FastQC (version 0.11.5)