FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34832559
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG527020.15130097102541332No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTCA951200.080.683379
TCTCTCA478250.078.214739
ATTCTCA636200.077.727069
TGTCTCA760500.077.56829
GTTCTCA532750.076.9459849
TATCTCA543900.076.579679
CTTCTCA420050.074.4627469
GGTCTCA383750.073.15649
GCTCTCA240300.071.483329
AGTCTCA335150.071.211499
ACTCTCA290250.069.9577949
TTTTCTC386950.068.9929358
CGTCTCA291650.067.567419
CATCTCA198150.067.065019
TATTCTC285250.066.163228
AATCTCA334750.066.116919
TTTTCCG216250.065.6965269
GGTTCCG242150.065.198339
TTATCTC251200.064.947148
CGTTCCG157900.064.620799
Produced by FastQC (version 0.11.5)