FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10592606
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTT175500.021.4900671
CTTGAAA129150.021.4293142
TACACGA178550.021.4187411
ACGAAGT292500.021.3957353
GCGCGAT170800.021.3535331
CACAAGA156700.021.3120292
GACGGCT201150.021.3040161
TGACTAG131050.021.2737311
GGGATGC157650.021.2463992
ACTTGTG235800.021.2302472
CCTACCA131050.021.2149641
CACGAAG295100.021.2097282
GTAGCCT230750.021.1866668
CAAGAGA158400.021.1780244
AGGGATG162300.021.129651
CTTGTGT236850.021.124353
TCACAAG211950.021.1164821
CACTCCT157650.021.103969
AGGCCCT174400.021.0888258
GAAGTAG178800.021.0746635
Produced by FastQC (version 0.11.5)