FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38294520
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG578300.1510137742945988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTCA1064950.080.682759
TCTCTCA529900.078.476319
ATTCTCA696750.077.81149
GTTCTCA576850.077.570389
TGTCTCA828400.077.486069
TATCTCA604200.076.8655249
CTTCTCA466100.074.547569
GGTCTCA419200.072.9932869
GCTCTCA266550.071.874849
AGTCTCA369600.071.577929
ACTCTCA323150.069.793769
TTTTCTC435800.068.720888
CATCTCA214550.067.2683649
AATCTCA372850.066.9532249
TATTCTC317350.066.864378
TTATCTC280300.066.7115868
CGTCTCA322400.066.647489
GGTTCCG263700.066.301889
ATTTCTC312950.065.265028
CGTTCCG171150.064.709669
Produced by FastQC (version 0.11.5)