FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38294520
Sequences flagged as poor quality0
Sequence length28
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC6397200.021.8508241
CCACACT6368750.021.8124182
ATTTCTG2034950.021.6932141
TCTGAGC1982000.021.6810134
AGCTGAA2008050.021.6515948
TGAGCTG1977050.021.6505836
ACACTCT6428750.021.626784
GAGCTGA1991200.021.61557
CTGAGCT1985600.021.6105385
TTTCTGA1982250.021.610052
TTCTGAG1999100.021.599063
CTTCATG6463950.021.5806839
GCTGAAA2004150.021.5510239
TCTTCAT6467400.021.54748
CTGAAAT2004550.021.54340410
CTCTTCA6441600.021.5368797
ACTCTTC6447450.021.5229666
CACTCTT6452700.021.4846575
CACACTC6489750.021.4706763
GCAGAGA1059850.021.4635494
Produced by FastQC (version 0.11.5)