FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16730266
Sequences flagged as poor quality0
Sequence length91
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT259038215.483208694948425No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT4843372.894974891612602No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT3437302.0545399577030037No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC1768611.057132026472263No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1665160.9952979827098983No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1486400.8884497114391368No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC1324000.7913801250978316No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA1269810.758989725566826No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT1107860.6621891128329939No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT654500.39120716909103537No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT639320.3821337927322853No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC589290.35222990477258403No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA484560.2896307805267412No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC412510.24656511737470282No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG398540.23821498116049084No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG392040.23432980683032772No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGT346070.20685265852915907No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC328870.19657188953241989No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC296440.17718785822054473No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT264780.15826407063701198No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC252430.15088223940970213No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG239170.14295648377616949No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG215730.12894594742247373No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG211640.1265012761901096No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT209730.12535963265616934No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA209440.12518629410913132No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGCTTGGAGGGTGTGGTGGT207240.12387131202815305No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG178680.10680045374054424No Hit
CTGCTGGCCGCGTACTTGTTGTTGTTTTGTTTGGAGGGTGTGGTGGTCTC173470.1036863370851366No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG171270.10237135500415834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT2817150.084.825351
CGTTATC2864500.083.404162
TTATCCA3008050.079.379184
CACGCTG179950.078.117861
TATCCAC3101750.077.5128565
GTTATCC3105700.076.995543
ATCCACC3134150.076.875636
CACCTTC3193250.075.286479
TCCACCT3380650.071.202367
GCGTTAA5150.071.048241
CCACCTT3416400.070.410018
GCGGTAT26000.065.128621
GACGTTT19650.064.4514543
AAGCAGT26550.064.099941
CGGTATC26550.063.5514952
GGGAAGT829250.062.8408131
GGTATCC27400.062.8179133
ACGTTTC20100.062.7970734
GGAAGTT821750.062.4366072
TTCTCGT11250.062.401281
Produced by FastQC (version 0.11.5)