FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38728827
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG596940.15413325066622854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTCA1069100.080.7131969
TCTCTCA537800.078.419839
ATTCTCA700300.077.863939
TGTCTCA833950.077.554329
GTTCTCA590300.077.50719
TATCTCA605550.076.3552259
CTTCTCA470600.074.555159
GGTCTCA423500.072.792079
GCTCTCA270800.071.7806559
AGTCTCA369000.070.598279
ACTCTCA334500.069.723299
TTTTCTC430800.068.4610758
CATCTCA220350.067.5017249
CGTCTCA327150.067.3927159
TATTCTC310800.067.1504448
AATCTCA389050.066.566699
ATTTCTC328750.064.983498
GGTTCCG271450.064.767279
TTATCTC285150.064.6213158
TTCTCTC261200.064.575518
Produced by FastQC (version 0.11.5)