FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16673888
Sequences flagged as poor quality0
Sequence length28
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACTT273950.021.4644221
CTTGAAA209800.021.4372842
TACACGA279600.021.4084051
ACGAAGT457850.021.3794363
GCGCGAT271450.021.3743571
CACGAAG460000.021.2761332
CAAGAGA249800.021.252714
CACAAGA248650.021.2197072
CTTGTGT363800.021.1986733
CCTACCA217500.021.1932491
ACTTGTG364550.021.1805952
GACGGCT315800.021.176921
TCACAAG331450.021.1727521
GTGTCAA302750.021.1608648
CACTCCT250300.021.1263879
CGAGCCT203400.021.105992
TGACTAG207500.021.0906281
AGGGATG249200.021.0887071
GGGATGC244400.021.0756552
GTAGCCT358150.021.0751518
Produced by FastQC (version 0.11.5)