FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34455264
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG524590.1522524976154587No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCTCA936050.080.910349
TCTCTCA493750.078.248429
ATTCTCA642850.077.9823159
TGTCTCA734700.077.5287869
GTTCTCA515900.077.1789259
TATCTCA538000.077.168289
CTTCTCA418550.073.969599
GGTCTCA377250.072.7961969
GCTCTCA240000.071.8397459
AGTCTCA329250.071.1337369
ACTCTCA290650.070.023779
TTTTCTC390600.068.316988
AATCTCA333600.067.314379
TATTCTC290750.067.3101048
CATCTCA193300.067.232099
CGTCTCA282550.066.6766059
TTATCTC256450.065.8891758
ATTTCTC281900.065.202078
TTTTCCG217250.065.160729
GGTTCCG234200.065.090199
Produced by FastQC (version 0.11.5)