FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005395143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005395143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34455264
Sequences flagged as poor quality0
Sequence length28
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCACAC5745650.021.8423561
CCACACT5717250.021.8011052
ATTTCTG1834250.021.7068561
TCTGAGC1789400.021.6883454
TGAGCTG1787500.021.6455546
AGCTGAA1812750.021.6231528
ACACTCT5768800.021.6188434
CTGAGCT1795450.021.6079125
GAGCTGA1793700.021.6050457
TTCTGAG1805750.021.594923
TTTCTGA1792250.021.5857622
CTTCATG5793450.021.583229
CTGAAAT1801850.021.57691610
CTCTTCA5774500.021.5487047
TCTTCAT5798300.021.5454358
GCTGAAA1806450.021.5335459
ACTCTTC5781500.021.53336
CACTCTT5788250.021.4865265
CACACTC5825850.021.4607623
GCAGAGA953800.021.43264
Produced by FastQC (version 0.11.5)